Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient

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Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient.

Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.

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Separation of cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns.

A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological studies.

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Preparation of synaptoneurosomes from mouse cortex using a discontinuous percoll-sucrose density gradient.

Synaptoneurosomes (SNs) are obtained after homogenization and fractionation of mouse brain cortex. They are resealed vesicles or isolated terminals that break away from axon terminals when the cortical tissue is homogenized. The SNs retain pre- and postsynaptic characteristics, which makes them useful in the study of synaptic transmission. They retain the molecular machinery used in neuronal si...

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enrichment of undifferentiated type a spermatogonia from goat testis using discontinuous percoll density gradient and differential plating

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1986

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.23.1.199-200.1986